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It depends what you mean. Testosterone makes a difference. It improves blood oxygen levels and cardio capacity, and it increases strength (it's a steroid).
And depending on the context, the difference is larger. Men tend to have stronger hand grip than women for example, to the extent, there is very little overlap. But if you look at leg strength, the distributions are different, but they overlap significantly. And when you start to look at things like long distance endurance events, the distributions are even closer.
However, none of those things align with "men" or "women". They align with "the dominant sex hormone in your body", and your capacity shifts as your sex hormones shift.