• The episode title calls back to the VOY season two episode, “Tuvix”, in which Neelix and Tuvok are combined into a single being by due to the unique properties of an alien orchid and the transporter.

• This episode was written by series creator Mike McMahan.

• Boimler [Jack Quaid] has holodeck waste removal duty, a task Mariner [Tawny Newsome] was assigned in “Moist Vessel” as part of a plan to get her to transfer off the USS Cerritos.

• It’s the USS Voyager! From Star Trek!

     • The VOY theme plays as we see the ship, and again later when the ship is landed on Earth.

• Voyager has been transformed into a museum piece. We see the ship displayed at the fleet museum in “The Bounty” some 20 years later. Exhibits on the ship commemorating the Voyager crew’s adventures include:

     • The Borg regeneration alcoves in Cargo Bay 2. One of the Cargo Bay 2s, anyway. - Established in “Scorpion, Part II”

     • The galley converted from what would have been the captain’s private dining hall, including authentic Talaxian foodstuffs.

     • The bridge.

     • The ”Neelix cheese”. - From “Learning Curve”

     • Janeway and Tom Paris’ hyper-evolved salamander forms after travelling at Warp 10. - Seen in “Threshold”

• The large battle in the title sequence has been updated again. In addition to Borg Cubes, Romulan Warbirds (season one), Klingon Birds-of-Prey, Pakled Clumpships (season two), and Crystalline Entity (season three), there is now a Breen Interceptor, and the Whale Probe introduced in “Star Trek: The Voyage Home”.

     • We can hear the Whale Probe’s call before the Cerritos warps away.

• Cap’n Freeman records the stardate as 58724.3 in her log.

• Tendi [Noël Wells] is moving a containment unit holding the orchid introduced in “Tuvix” when the lid pops off and a petal floats through the ventilation system into the transporter room.

• Billups tells Doctor T’Ana about the pet dragon he had growing up. Billups was established as coming from the Hysperian colony settled by “Ren faire type” people in “Where Pleasant Fountains Lie”

• Billups and Doctor T’Ana are combined into a single being calling himself T’Illups. Much like Tuvix, the yoke of T’Illups’ uniform has a floral pattern.

• Throughout the episode, more characters get Tuvixed:

     • Cap’n Freeman and Doctor Migleemo - Captain Doctor Frigleeman

     • Shaxs and Barnes - Shabarnes

     • Bartender Honus and Transporter Chief Lundy - Chondus

     • Matt the whale and Steve Stevens - Swhale Swhalens

     • Nurse Westlake and Jennifer - We don’t actually see the combined form

• ”She knows Janeway straight up murdered Tuvix, right?” This is an accurate description of the conclusion of that episode.

• Mariner accidently opens a panel on the Voyager bridge, releasing one of the Tak Takian macroviruses introduced in “Macrocosm”.

• “Uh, you know, Chakotay served here.” Technically true up until about season five of VOY, at which point the only characters aboard the ship were Seven of Nine, the Doctor, and sometimes Captain Janeway.

• ”Dude, this is nothing compared to, you know, that Pike thing we aren’t supposed to talk about.” Mariner is referring to the events of “Those Old Scientists”.

• ”How many…physical memories do you have from before?” Shaxs and Doctor T’Ana have an intimate relationship, implied at least as far back as "Mugato, Gumato".

• One of the macroviruses impacts a panel, causing it to create holograms of Doctor Chaotica from “Night”, the Clown from “The Thaw”, and Michael Sullivan from “Fairhaven”.

     • While Chaotica and Sullivan were holodeck characters created by Tom Paris, the Clown was a manifestation of the fears of five aliens neurally linked together in stasis. Mariner does point out that the Clown wasn’t a holodeck program.

     • Martin Rayner, Michael McKean, and Fintan McKeown are not credited for the episode, so it would seem that none of them are reprising their roles.

• Beljo Tweekle installed holo-emitters throughout the ship. In “The Killing Game” the Hirogen did the same, for the purposes of their wargame simulations with the Voyager crew.

• One of the marcoviruses has Harry Kim’s clarinet. It was established in “Caretaker” that Kim played the instrument, though he gave it up in favour of the saxophone by season six’s “Ashes to Ashes”.

• A Borg nanite attempts to assimilate to macrovirus, becoming a macronanite.

• “Computer, delete this guy! Come on, computer!” In “Fairhaven” Janeway uttered her famous line, “Delete the wife,” regarding Sullivan’s spouse.

     • “I miss my wife.” Apparently at some point Sullivan’s memories of his wife were restored to him, or he remarried.

• Boimler is concerned that if he’s promoted, it will negatively impact his relationship with Mariner, just as it did when he accepted the promotion to the USS Titan and left without telling any of his friends, or answering Mariner’s messages, in “No Small Parts”.

• Mariner was sent to Starbase 80 in “Trusted Sources”.

• T’Lyn is able to combine all the Tuvixed beings into one creature, which is then described by Tendi as a “Non-sentient blob of meat,” handily circumventing the ethical dilemma presented by “Tuvix”.

• Boimler claims to be the son of Captain Proton, the character whom Tom Paris played in these simulations, and Doctor Chaotica’s mortal enemy. First seen in “Night”.

• Rutherford [Eugene Cordero] gums up Voyager with the brill cheese as it did on it’s own in “Learning Curve”.

• Boimler, T’Lyn, Tendi, and Mariner all get promoted to lieutenant junior grade. Mariner was briefly a full lieutenant in “Moist Vessel”, and Boimler was lieutenant junior grade while serving aboard the Titan in “No Small Parts”, “Strange Energies” and “Kayshon, His Eyes Open”.

• ”My main objective here is to prove to the High Council that I should be reinstated to the Vulcan fleet.” T’Lyn was believes her provisional assignment to Starfleet is an unwarranted punishment, as per “wej Duj”.

• It’s the IKS Che’Ta’! From Star Trek! Specifically from “wej Duj”

     • The Mysterious Threat destroys the Che’Ta’, but even though we see a close up of the wreckage, including a spear and bloodwine barrel, but no bodies.